Novel pathogenic characteristics of highly pathogenic avian influenza virus H7N9: viraemia and extrapulmonary infection.
Identifieur interne : 000324 ( Main/Exploration ); précédent : 000323; suivant : 000325Novel pathogenic characteristics of highly pathogenic avian influenza virus H7N9: viraemia and extrapulmonary infection.
Auteurs : Xiao-Xin Wu [République populaire de Chine] ; Ling-Zhai Zhao [République populaire de Chine] ; Song-Jia Tang [République populaire de Chine] ; Tian-Hao Weng [République populaire de Chine] ; Wei-Gen Wu [République populaire de Chine] ; Shu-Hao Yao [République populaire de Chine] ; Hai-Bo Wu [République populaire de Chine] ; Lin-Fang Cheng [République populaire de Chine] ; Jian Wang [République populaire de Chine] ; Feng-Yu Hu [République populaire de Chine] ; Nan-Ping Wu [République populaire de Chine] ; Hang-Ping Yao [République populaire de Chine] ; Fu-Chun Zhang [République populaire de Chine] ; Lan-Juan Li [République populaire de Chine]Source :
- Emerging microbes & infections [ 2222-1751 ] ; 2020.
Abstract
The H7N9 virus mutated in 2017, resulting in new cases of highly pathogenic avian influenza (HPAI) H7N9 virus infection. H7N9 was found in a viraemic patient in Guangdong province, China. The present study aimed to clarify the pathogenic characteristics of HPAI H7N9. Virus was isolated from the plasma and sputum of the patient with HPAI H7N9. Liquid phase chip technology was used to detect the plasma cytokines from the infected patient and healthy controls. Mice were infected with strains A/Guangdong/GZ8H002/2017(H7N9) and A/Zhejiang/DTID-ZJU01/2013(H7N9) to observe the virus's pathogenic characteristics. Serum and brain tissue were collected at 2, 4, and 6 days after infection. The viruses in serum and brain tissue were detected and isolated. The two strains were infected into A549 cells, exosomes were extracted, and virus genes in the exosomes were assessed. Live virus was isolated from the patient's plasma. An acute cytokine storm was detected during the whole course of the disease. In animal experiments, A/Guangdong/GZ8H002/2017(H7N9) was more pathogenic than A/Zhejiang /DTID-ZJU01/2013(H7N9) and resulted in the death of mice. Live virus was isolated from infected mouse serum. Virus infection was also detected in the brain of mice. Under viral stress, A549 cells secreted exosomes containing the entire viral genome. The viraemic patient was confirmed to have an HPAI H7N9 infection. A/Guangdong/GZ8H002/2017(H7N9) showed significantly enhanced toxicity. Patient deaths might result from cytokine storms and brain infections. Extrapulmonary tissue infection might occur via the exosome pathway. The determined pathogenic characteristics of HPAI H7N9 will contribute to its future treatment.
DOI: 10.1080/22221751.2020.1754135
PubMed: 32267217
Affiliations:
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wicri:level="1"><nlm:affiliation>Plastic and Aesthetic Surgery Department, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 31000, Zhejiang, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plastic and Aesthetic Surgery Department, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 31000, Zhejiang</wicri:regionArea><wicri:noRegion>Zhejiang</wicri:noRegion></affiliation></author><author><name sortKey="Weng, Tian Hao" sort="Weng, Tian Hao" uniqKey="Weng T" first="Tian-Hao" last="Weng">Tian-Hao Weng</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, 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310003</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Cheng, Lin Fang" sort="Cheng, Lin Fang" uniqKey="Cheng L" first="Lin-Fang" last="Cheng">Lin-Fang Cheng</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang 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Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Nan Ping" sort="Wu, Nan Ping" uniqKey="Wu N" first="Nan-Ping" last="Wu">Nan-Ping Wu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for 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Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Zhao, Ling Zhai" sort="Zhao, Ling Zhai" uniqKey="Zhao L" first="Ling-Zhai" last="Zhao">Ling-Zhai Zhao</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Tang, Song Jia" sort="Tang, Song Jia" uniqKey="Tang S" first="Song-Jia" last="Tang">Song-Jia Tang</name><affiliation wicri:level="1"><nlm:affiliation>Plastic and Aesthetic Surgery Department, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 31000, Zhejiang, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Plastic and Aesthetic Surgery Department, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou 31000, Zhejiang</wicri:regionArea><wicri:noRegion>Zhejiang</wicri:noRegion></affiliation></author><author><name sortKey="Weng, Tian Hao" sort="Weng, Tian Hao" uniqKey="Weng T" first="Tian-Hao" last="Weng">Tian-Hao Weng</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Wu, Wei Gen" sort="Wu, Wei Gen" uniqKey="Wu W" first="Wei-Gen" last="Wu">Wei-Gen Wu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Yao, Shu Hao" sort="Yao, Shu Hao" uniqKey="Yao S" first="Shu-Hao" last="Yao">Shu-Hao Yao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Stormotologry, Wenzhou Medical University Renji College, Wenzhou 325035, Zhejiang, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Stormotologry, Wenzhou Medical University Renji College, Wenzhou 325035, Zhejiang</wicri:regionArea><wicri:noRegion>Zhejiang</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Hai Bo" sort="Wu, Hai Bo" uniqKey="Wu H" first="Hai-Bo" last="Wu">Hai-Bo Wu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Cheng, Lin Fang" sort="Cheng, Lin Fang" uniqKey="Cheng L" first="Lin-Fang" last="Cheng">Lin-Fang Cheng</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Hu, Feng Yu" sort="Hu, Feng Yu" uniqKey="Hu F" first="Feng-Yu" last="Hu">Feng-Yu Hu</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Wu, Nan Ping" sort="Wu, Nan Ping" uniqKey="Wu N" first="Nan-Ping" last="Wu">Nan-Ping Wu</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Yao, Hang Ping" sort="Yao, Hang Ping" uniqKey="Yao H" first="Hang-Ping" last="Yao">Hang-Ping Yao</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Zhang, Fu Chun" sort="Zhang, Fu Chun" uniqKey="Zhang F" first="Fu-Chun" last="Zhang">Fu-Chun Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Infectious Diseases, Guangzhou Eighth People's Hospital, Guangzhou Medical University, Guangzhou 510060, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Li, Lan Juan" sort="Li, Lan Juan" uniqKey="Li L" first="Lan-Juan" last="Li">Lan-Juan Li</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Centre for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author></analytic><series><title level="j">Emerging microbes & infections</title><idno type="eISSN">2222-1751</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The H7N9 virus mutated in 2017, resulting in new cases of highly pathogenic avian influenza (HPAI) H7N9 virus infection. H7N9 was found in a viraemic patient in Guangdong province, China. The present study aimed to clarify the pathogenic characteristics of HPAI H7N9. Virus was isolated from the plasma and sputum of the patient with HPAI H7N9. Liquid phase chip technology was used to detect the plasma cytokines from the infected patient and healthy controls. Mice were infected with strains A/Guangdong/GZ8H002/2017(H7N9) and A/Zhejiang/DTID-ZJU01/2013(H7N9) to observe the virus's pathogenic characteristics. Serum and brain tissue were collected at 2, 4, and 6 days after infection. The viruses in serum and brain tissue were detected and isolated. The two strains were infected into A549 cells, exosomes were extracted, and virus genes in the exosomes were assessed. Live virus was isolated from the patient's plasma. An acute cytokine storm was detected during the whole course of the disease. In animal experiments, A/Guangdong/GZ8H002/2017(H7N9) was more pathogenic than A/Zhejiang /DTID-ZJU01/2013(H7N9) and resulted in the death of mice. Live virus was isolated from infected mouse serum. Virus infection was also detected in the brain of mice. Under viral stress, A549 cells secreted exosomes containing the entire viral genome. The viraemic patient was confirmed to have an HPAI H7N9 infection. A/Guangdong/GZ8H002/2017(H7N9) showed significantly enhanced toxicity. Patient deaths might result from cytokine storms and brain infections. Extrapulmonary tissue infection might occur via the exosome pathway. The determined pathogenic characteristics of HPAI H7N9 will contribute to its future treatment.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement></list><tree><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Wu, Xiao Xin" sort="Wu, Xiao Xin" uniqKey="Wu X" first="Xiao-Xin" last="Wu">Xiao-Xin Wu</name></region><name sortKey="Cheng, Lin Fang" sort="Cheng, Lin Fang" uniqKey="Cheng L" first="Lin-Fang" last="Cheng">Lin-Fang Cheng</name><name sortKey="Hu, Feng Yu" sort="Hu, Feng Yu" uniqKey="Hu F" first="Feng-Yu" last="Hu">Feng-Yu Hu</name><name sortKey="Li, Lan Juan" sort="Li, Lan Juan" uniqKey="Li L" first="Lan-Juan" last="Li">Lan-Juan Li</name><name sortKey="Tang, Song Jia" sort="Tang, Song Jia" uniqKey="Tang S" first="Song-Jia" last="Tang">Song-Jia Tang</name><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name><name sortKey="Weng, Tian Hao" sort="Weng, Tian Hao" uniqKey="Weng T" first="Tian-Hao" last="Weng">Tian-Hao Weng</name><name sortKey="Wu, Hai Bo" sort="Wu, Hai Bo" uniqKey="Wu H" first="Hai-Bo" last="Wu">Hai-Bo Wu</name><name sortKey="Wu, Nan Ping" sort="Wu, Nan Ping" uniqKey="Wu N" first="Nan-Ping" last="Wu">Nan-Ping Wu</name><name sortKey="Wu, Wei Gen" sort="Wu, Wei Gen" uniqKey="Wu W" first="Wei-Gen" last="Wu">Wei-Gen Wu</name><name sortKey="Yao, Hang Ping" sort="Yao, Hang Ping" uniqKey="Yao H" first="Hang-Ping" last="Yao">Hang-Ping Yao</name><name sortKey="Yao, Shu Hao" sort="Yao, Shu Hao" uniqKey="Yao S" first="Shu-Hao" last="Yao">Shu-Hao Yao</name><name sortKey="Zhang, Fu Chun" sort="Zhang, Fu Chun" uniqKey="Zhang F" first="Fu-Chun" last="Zhang">Fu-Chun Zhang</name><name sortKey="Zhao, Ling Zhai" sort="Zhao, Ling Zhai" uniqKey="Zhao L" first="Ling-Zhai" last="Zhao">Ling-Zhai Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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